pcdna empty vector control (pcdna)  (Shanghai GenePharma)

Journal: Experimental and Therapeutic Medicine

Article Title: MicroRNA-195-5p suppresses the proliferation, migration, invasion and epithelial-mesenchymal transition of laryngeal cancer cells in vitro by targeting E2F3

doi: 10.3892/etm.2021.10512

Figure Lengend Snippet: miR-195-5p suppresses the proliferation, migration and invasion of AMC-HN-8 cells by regulating E2F3 expression. (A) E2F3 overexpression efficiency was detected by western blot analysis. (B) miR-195-p mimic and pcDNA-E2F3 were co-transfected into AMC-HN-8 cells and cell proliferation was detected by Cell Counting Kit-8 assays. (C and D) Cell migration in each group following transfection with miR-195-5p and pcDNA-E2F3 was detected by wound-healing assays. Magnification, x100. (E and F) Cell invasion in each group following transfection with miR-195-5p and pcDNA-E2F3 was detected by Transwell assays. Magnification, x100. (G) Western blot analysis and (H) RT-qPCR assay were respectively used to measure the protein and mRNA levels of the epithelial-mesenchymal transition-associated proteins (E-cadherin, vimentin, N-cadherin and snail) and MMP-2 and -9. ** P<0.01; *** P<0.001. miR, microRNA; E2F3, E2F transcription factor 3; miR, microRNA; NC, negative control; MMP, matrix metalloprotease.

Article Snippet: The E2F3 overexpression vector pcDNA-E2F3 and empty control vector pcDNA-NC were constructed by Shanghai GenePharma Co., Ltd.

Techniques: Migration, Expressing, Over Expression, Western Blot, Transfection, Cell Counting, Quantitative RT-PCR, Negative Control

Journal: Translational Oncology

Article Title: Upregulation of miR-142-3p Improves Drug Sensitivity of Acute Myelogenous Leukemia through Reducing P-Glycoprotein and Repressing Autophagy by Targeting HMGB1

doi: 10.1016/j.tranon.2017.03.003

Figure Lengend Snippet: HMGB1 reversed miR-142-3p–induced drug sensitivity in HL-60/ATRA and HL-60/ADR cells. (A and B) After cells treated with ATRA or ADR were transfected with miR-142-3p, miR-control or along with pcDNA-HMGB1 or vector, cell viability was determined by MTT assay. (C and D) After cells treated with ATRA or ADR were transfected with miR-142-3p, miR-control or along with pcDNA-HMGB1 or vector, apoptosis was examined by flow cytometry. * P < .05.

Article Snippet: miR-142-3p mimics (miR-142-3p), anti–miR-142-3p, miRNA control (miR-control), pcDNA-HMGB1, and pcDNA empty control (vector) were purchased from Genepharma (Shanghai, China).

Techniques: Transfection, Plasmid Preparation, MTT Assay, Flow Cytometry

Journal: Translational Oncology

Article Title: Upregulation of miR-142-3p Improves Drug Sensitivity of Acute Myelogenous Leukemia through Reducing P-Glycoprotein and Repressing Autophagy by Targeting HMGB1

doi: 10.1016/j.tranon.2017.03.003

Figure Lengend Snippet: miR-142-3p upregulation enhanced drug sensitivity of AML cells through reducing P-gp and suppressing autophagy by targeting HMGB1. The level of P-gp in HL-60/ATRA (A) and HL-60/ADR (B) cells transfected with miR-142-3p or miR-control, or together with pcDNA-HMGB1 or vector were detected by Western blot. The levels of autophagy related proteins Atg5, LC3-I, and LC3-II were analyzed by Western blot in HL-60/ATRA (C) and HL-60/ADR (D) cells transfected with miR-142-3p or miR-control, or together with pcDNA-HMGB1 or vector. * P < .05.

Article Snippet: miR-142-3p mimics (miR-142-3p), anti–miR-142-3p, miRNA control (miR-control), pcDNA-HMGB1, and pcDNA empty control (vector) were purchased from Genepharma (Shanghai, China).

Techniques: Transfection, Plasmid Preparation, Western Blot